Introduction and objective: The work of flight attendants is associated with exposure to disturbances in the circadian rhythm, sleep deficiency or deprivation, and irregular meal consumption. These factors may lead to disrupted leptin secretion and an increased risk of carbohydrate metabolism disorders. The aim of the study was to determine the level of leptin, glucose and insulin patterns and to seek factors of potential influence on the secretion of leptin, glucose and insulin in female flight attendants working within one time zone (more take-offs and more landings) as well as on long-distance flights. Material and methods: The cross-sectional study involved 103 Polish women aged 23–46, divided into two groups: Ia - flying within one time zone and group Ib - stewardesses working on long-distance flights. The control group (II) comprised women seeking help for marital infertility (male factor). Variables assessed included age, BMI, menstrual cycle regularity, service length, flying frequency, leptin concentration, OGTT, insulin pattern, and HOMA-IR. Descriptive and inferential statistical methods were used for data analysis. Results: The incidence of hyperleptinemia in female flight attendants (22%) was significantly higher than in the control group (6%), p <0,05. Groups Ia and Ib in individual concentrations were not statistically significant (p=0.118). Impaired Glucose Tolerance (IGT) was found in 10% of the study group and 5% of women in the control group. Hyperinsulinemia occurred slightly more frequently in flight attendants, especially in those flying on long-distance flights, working less frequently, and having longer work experience Conclusions: The specific working conditions of flight attendants, especially their shift work nature, contribute to a higher prevalence of hyperleptinemia. Considering the fact that Impaired Glucose Tolerance occurs two times more frequently in flight attendants, it is reasonable to assume that this occupational group is at risk of developing diabetes in the future.